We show time-dependent perturbation of the metabolism of amino acids, nucleotides, fatty acids, tricarboxylic acids, photosynthesis and photorespiration by both Ag-treatments. We combine targeted metabolomics, biouptake and physiological response studies to elucidate metabolic perturbations in alga Poterioochromonas malhamensis induced by AgNPs and dissolved Ag. Despite progress in assessing their environmental implications, knowledge gaps exist concerning the metabolic perturbations induced by AgNPs on phytoplankton, essential organisms in global biogeochemical cycles and food-web dynamics. 8W.M.Silver nanoparticles (AgNPs) are one of the most used engineered nanomaterials. 5Department of Morphology and Molecular Pathology, 6Department of Abdominal Surgery, and 7Department of Gastroenterology, University Hospitals Leuven, Leuven, Belgium.

4IFOM, FIRC Institute of Molecular Oncology, Milano, Italy. 3Department of Inflammation and Immunology, Humanitas Clinical and Research Center, Rozzano, Milano, Italy. 5165 E-mail: note: Alessandra Villa and Francesca Angiolini contributed equally to this work.ġDepartment of Experimental Oncology and 2Molecular Medicine Program, European Institute of Oncology, Milano, Italy. 10Department of Oncology, Katholieke Universiteit (KU) Leuven, Leuven, Belgium.Īddress correspondence to: Ugo Cavallaro, Molecular Medicine Program, European Institute of Oncology, Via G. 9Laboratory of Molecular Oncology and Angiogenesis, Vesalius Research Center, Vlaams Instituut voor Biotechnologie (VIB), Leuven, Belgium. Keck Center for Collaborative Neuroscience and Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, New Jersey, USA.

1Department of Experimental Oncology and 2Molecular Medicine Program, European Institute of Oncology, Milano, Italy.

Normalization in graphpad prism 8